Turf stockpiling, a prevalent but overlooked anthropogenic disturbance, poses significant threats to fragile meadow ecosystems. However, the consequences of turf stockpiling on ecosystem multifunctionality remain poorly understood, especially across trophic levels in alpine ecosystems. Here, we assessed the biodiversity-function relationships following a two-year turf stockpiling test in alpine meadows. Nineteen indicators were selected to quantify the functioning related to biomass production, organic matter decomposition, and nutrient cycling as well as to ecosystem multifunctionality. Plant diversity decreased in laid turfs but increased in stacked turfs compared to ambient meadows, while most microbial diversity and network complexity reduced in both stockpiled turfs. Laid turfs and stacked turfs exerted neutral and positive effects on ecosystem multifunctionality, respectively, as a result of the trade-off between above- and below-ground functions. Ecosystem multifunctionality negatively related to soil pH, the average degree of bacterial network, and microbial β diversity, but positively related plant richness. Bacterial β diversity and the average degree of bacterial network, and soil pH were crucial factors predicting ecosystem multifunctionality as well as the functions relative to organic matter decomposition and nutrient cycling. Microbial network complexity contributed most (58.1 %) to variation in multifunctionality than microbial α (11.9 %) and β (20.0 %) diversity. The findings revealed that bacteria, rather than fungi or plants, predominantly drove ecosystem multifunctionality in the disturbed meadows, particularly bacterial network complexity. We suggested that optimizing turf stockpiling practices to preserve these complex microbial associations should be a priority for effective ecological restoration.