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简介： 
吴中柳研究员，女，1975年6月生。2003年毕业于中国科学院上海有机化学研究所生物有机化学国家重点实验室，获理学博士学位，同年赴美国Vanderbilt大学医学院生物化学系从事博士后研究工作，2006年入选中国科学院“百人计划”(“引进国外杰出人才”) ，2008年获得择优支持。现任中国科学院成都生物研究所应用与环境微生物研究中心研究员，博士生导师。入选2008年度四川省杰出青年学科带头人。
主要从事生物催化与生物转化研究。包括：1、新型微生物氧化还原酶酶源的筛选、评估和应用；2、酶催化剂的分子改造和进化；3、重要手性分子的酶催化合成新方法。4、生物催化法构建多样性的活性分子库和新型类药分子。
主持项目：
国家自然科学基金21072183/B020104 (2011.1-2013.12)
国家自然科学基金青年项目20802073/B020104 (2009.1 - 2011.12)
中科院百人计划 (2009.1 - 2011.12)
中国科学院知识创新工程重大项目专题KSCX1-YW-11B2 (2008.1 - 2011.12)
四川省青年科技基金 08ZQ026-023 (2008.1 - 2011.1)
主要论著：
1. Zhang, S.-B.; Ma, X.-F.; Pei, X.-Q.; Liu, J.-Y.; Shao, H.-W.*, Wu, Z.-L.* A practical high-throughput screening system for feruloyl esterases: substrate design and evaluation. J. Mol. Catal. B: Enzym. 2011, 10.1016/j.molcatb.2011.08.011.
2. Lin, H.; Liu, J.-Y.; Wang, H.-B.; Ahmed, A. A. Q.; Wu, Z.-L.* Biocatalysis as an alternative for the production of chiral epoxides: a comparative review. J. Mol. Catal. B: Enzym. 2011, 72(3-4), 77-89. (Invited review)
3. Lin, H.; Liu, Y.; Wu, Z.-L.* Highly diastereo- and enantio-selective epoxidation of secondary allylic alcohols catalyzed by styrene monooxygenase. Chem. Commun., 2011, 47, 2610-2612.
4. Tang, C.-G.; Lin, H.; Zhang, C.; Liu, Z.-Q.; Yang, T.; Wu, Z.-L.* Highly enantioselective bioreduction of N-methyl-3-oxo-3- (thiophen-2-yl) propanamide for the production of (S)-duloxetine. Biotechnol. Lett. 2011, 33(7), 1435-1440.
5. Lin, H.; Liu, Y.; Wu, Z.-L.* Asymmetric epoxidation of styrene derivatives by styrene monooxygenase from Pseudomonas sp. LQ26: effects of a- and b-substituents. Tetrahedron: Asymmetry, 2011, 22, 134-137.
6. Yi, Z.-L.; Pei, X.-Q.; Wu, Z.-L.* Introduction of glycine and proline residues onto protein surface increases the thermostability of endoglucanase CelA from Clostridium thermocellum. Bioresour. Technol. 2011, 102(3), 3636–3638
7. Pei, X.-Q.; Yi, Z.-L.; Tang, C.-G.; Wu, Z.-L.* Three amino acid changes contribute markedly to the thermostability of b-glucosidase BglC from Thermobifida fusca. Bioresour. Technol. 2011, 102(3), 3337–3342.
8. Qaed, A. A.; Lin, H.; Tang, D.-F.; Wu, Z.-L.* Rational design of styrene monooxygenase mutants with altered substrate preference. Biotechnol. Lett. 2011, 33, 611-616.
9. Zhang, S.-B.; Wu, Z.-L.* Identification of amino acid residues responsible for increased thermostability of feruloyl esterase A from Aspergillus niger using the PoPMuSiC algorithm. Bioresour. Technol. 2011, 102 (2), 2093–2096.
10. Lin, H.; Qiao, J.; Liu, Y.; Wu, Z.-L.* Styrene monooxygenase from Pseudomonas sp. LQ26 catalyzes the asymmetric epoxidation of both conjugated and unconjugated alkenes. J. Mol. Catal. B: Enzym. 2010, 67(3-4), 236-241.
11. Zhang, Z.-G.; Yi, Z.-L.; Pei, X.-Q.; Wu, Z.-L.* Improving the thermostability of Geobacillus stearothermophilus xylanase XT6 by directed evolution and site-directed mutagenesis. Bioresour. Technol. 2010, 101(23), 9272-9278.
12. Yi, Z.-L.; Wu, Z.-L.* Mutations from a family-shuffling-library reveal amino acid residues responsible for the thermostability of endoglucanase CelA from Clostridium thermocellum Biotechnol. Lett. 2010, 32(12), 1869-1875.
13. Tang, Z., Salamanca-Pinzon, S.G., Wu, Z.L., Xiao, Y., Guengerich, F.P., Human cytochrome P450 4F11: Heterologous expression in bacteria, purification, and characterization of catalytic function. Arch. Biochem. Biophys. 2010, 494(1), 86-93.
14. Wu, Z.-L.*; Qiao, J.; Zhang, Z.-G.; Guengerich, F.P.; Liu, Yan; Pei, X.-Q. Enhanced bacterial expression of several mammalian cytochrome P450s by codon optimization and chaperone coexpression. Biotechnol. Lett. 2009, 31(10), 1589-1593.
15. Zhang, Z.-G.; Liu, Y.; Guengerich, F.P.; Matse, J.H.; Chen J.; Wu, Z.-L.* Identification of amino acid residues involved in 4-chloroindole 3-hydroxylation by cytochrome P450 2A6 using screening of random libraries. J. Biotechnol. 2009, 139(1), 12-18.
16. Stark, K.; Wu, Z.-L.; Bartleson, C.J.; Guengerich, F.P. Heterologous expression and mRNA distribution of orphan cytochrome P450 20A1. Drug Metab. Dispos. 2008, 36(9), 1930-1937.
17. Wu, Z.-L.; Sohl, C.D.; Shimada, T.; Guengerich, F.P. Recombinant Enzymes Over expressed in Bacteria Show Broad Catalytic Specificity of Human Cytochrome P450 2W1 and Limited Activity of Human Cytochrome P450 2S1. Mol. Pharmacol., 2006, 69(6), 2007-2014.
18. Wu, Z.-L.; Bartleson, C.J.; Ham, A.J.; Guengerich, F.P. Heterologous Expression, Purification, and Properties of human cytochrome P450 27C1, Arch. Biochem. Biophys. 2006, 445(1), 138-146.
19. Wu, Z.-L.; Podust, L.M.; Guengerich, F.P. Expansion of Substrate Specificity of Cytochrome P450 2A6 by Random and Site-Directed Mutagenesis. J. Biol. Chem. 2005, 280(49), 41090-41100.
20. Kim, D.; Wu, Z.-L.; Guengerich, F.P. Analysis of coumarin 7-hydroxylation activity of cytochrome P450 2A6 using random mutagenesis. J. Biol. Chem. 2005, 280(48),40319-40327.
21. Wu, Z.-L.; Aryal, P.; Lozach, O.; Meijer, L.; Guengerich, F.P. Biosynthesis of New Indigoid Inhibitors of Protein Kinases Using Recombinant Cytochrome P450 2A6, Chem. Biodiv. 2005, 2(1), 51-65.
22. Guengerich, F.P.; Wu, Z.-L.; Bartleson, C.J. Function of Human Cytochrome P450s: Characterization of the Orphans. Biochem. Biophys. Res. Commun. 2005, 338(1), 465-469.
23. Guengerich, F.P.; Wu, Z.-L. Biosynthesis of novel indirubins by recombinant cytochrome P450 systems. Invited chapter in Indirubin, the Red Shade of Indigo, edited by Meijer, L.; Guyard, N.; Skaltsounis, L; Eisenbrand, G. Editions <life in progress>, Roscoff, Fr. 2006, 79-87.
24. Wu, Z.-L.; Li, Z.-Y. Highly Enantioselective Synthesis of a,a-Disubstituted Malonamic Acids through Asymmetric Hydrolysis of Dinitriles with Rhodococcus sp. CGMCC 0497, Chem. Commun. 2003, 3, 386-387.(Selected by ChemInform, 2003, 34(23), Nitriles Q0520.)
25. Wu, Z.-L.; Li, Z.-Y. Practical Synthesis of Optically Active a,a-Disubstituted Malonamic Acids through Asymmetric Hydrolysis of Malonamide Derivatives with Rhodococcus sp. CGMCC 0497, J. Org. Chem. 2003, 68(6), 2479-2482.
26. Wu, Z.-L.; Li, Z.-Y. Biocatalytic Asymmetric Hydrolysis of (±)-b-Hydroxy Nitriles by Rhodococcus sp. CGMCC 0497, J. Mol. Catal. B: Enzym. 2003, 22(1-2), 105-112.
27. Wu, Z.-L.; Li, Z.-Y. Enantioselective Biotransformation of a,a-Disubstituted Dinitriles to the Corresponding 2-Cyano-Acetamides using Rhodococcus sp. CGMCC 0497, Tetrahedron: Asymmetry, 2003, 14(15), 2133-2142.
28. Wu, Z.-L.; Li, Z.-Y. Enhancement of Enzyme Activity and Enantioselectivity via Cultivation in Nitrile Metabolism by Rhodococcus sp. CGMCC 0497, Biotechnol. Appl. Biochem. 2002, 35(1), 61-67.
29. Wu, Z.-L.; Li, Z.-Y. Enantioselective Hydrolysis of Various Racemic alpha-Substituted Arylacetonitriles using Rhodococcus sp. CGMCC 0497, Tetrahedron: Asymmetry2001, 12(23), 3305-3312.
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